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KMID : 0848120110360020091
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International Journal of Oral Biology
2011 Volume.36 No. 2 p.91 ~ p.101
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Global Transcriptional Analysis Reveals Upregulation of NF--responsive and Interferon-stimulated Genes in Monocytes by Treponema lecithinolyticum Major Surface Protein
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Lee Sung-Hoon
Lee Hae-Ri
Jun Hye-Kyoung
Choi Bong-Kyu
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Abstract
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MspTL is the major surface protein of Treponema lecithinolyticum associated with periodontitis and endodontic infections. Our recent investigation revealed that MspTL induces proinflammatory cytokines and intercellular adhesion molecule 1 in THP-1 cells and periodontal ligament cells. In this study we conducted oligonucleotide microarray analysis to investigate the global transcriptional regulation in THP-1 cells stimulated with purified recombinant MspTL. MspTL upregulated the expression of 90 genes in THP-1 cells at least four fold, and the functions of these genes were categorized into adhesion, apoptosis/antiapoptosis, cell cycle/growth/differentiation, chemotaxis, cytoskeleton organization, immune response, molecular metabolism, proteolysis, signaling, and transcription. The majority of the modified genes are known to be NF--responsive and interferon-stimulated genes (ISGs). The expression of 12 selected genes was confirmed by real-time RT-PCR. Because prostaglandin is an important inflammatory mediator and Cox-2 was found to be induced by MspTL in the microarray analysis, we determined the level of in the culture supernatants of MspTL-treated cells and found that MspTL significantly increased . Our results provide insight into the gene regulation of host cells in response to MspTL, and may contribute to the understanding of the molecular mechanism in periodontitis.
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KEYWORD
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T. lecithinolyticum, Surface protein, Oligonulceotide microarray
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